Concanavalin A ( Con A ), is a kind of lectin separated from sword bean . A newly cloned lectin , FRIL ( Flt3 receptor-interacting lectin ), was identified to be capable of preserving primitive progenitors in vitro without loss of repopulating capacity .
When CD34 + cells were cultured in the presence of this lectin or its counterpart FL , within the 28 days of culture , FRIL can keep more cells in G0 / G1 phase ( beyond 80 % ) and 1 5 fold colony forming cells more than FL does after 14 day s culture .
The Mechanisms of A New Plant Lectin FRIL from Dolichos Lablab That Preserves Quiescent Hematopoietic Stem Cells in Suspension Culture
新的豆类凝集素FRIL及其体外维持造血干/祖细胞特性的作用机制
We have also found another receptor of FRIL by affinity purify and western blot with antibody to FRIL . It may be a new mediate to FRIL \'s function .
利用亲和纯化和自制受体的研究还发现FRIL可能存在Flt3之外的受体来介导它的功能。
But the underlying mechanism for maintaining quiescence of HSCs is largely unknown at present , so in this study , we focused on the mechanism of how FRIL maintains quiescence of HSCs through regulation of cell cycle .
但是其维持造血干祖细胞静息的机制目前还不清楚,因此本文从细胞周期调控的角度对这一问题进行了探讨。
To investigate the underlying mechanism of how FRIL maintain HSCs in vitro , we detected the expression of various cell cycle regulators , especially those involved in G0 / G1 phase regulation on mRNA and protein level .