Methods first , to acquire the highly pured glomeruli by three steps grade filtrated net ; next , to culture the microvascular endothelial cell in vitro with the transplant methods of isolated glomerali .
方法采用三步梯度筛网法,先获得较高纯度的肾血管球,再应用肾血管球外植法体外培养肾微血管内皮细胞。
Results immunochemistry analysis showed that non-muscle myosin heavy chain iia ( nmmhc-iia ) was expressed in podocytes of glomeruli and distal convoluted tube , and was faintly expressed in the brush border of proximal tube .