Epoxidized soybean oil with high epoxy value ( HESO ), prepared by epoxidation of natural soybean oil , is a kind of environmental protection PVC plasticizer . HESO is superior to existing ordinary epoxidized soybean oil ( ESO ) in performance , and can replace phthalate esters plasticizer.In this paper , through screening and optimization of HESO preparation process , the optimum process of two-step method was determined , and the effects of reaction time , reaction temperature , formic acid dosage , hydrogen peroxide dosage on the preparation of HESO were investigated .