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- 根据这一的特点，我们在腺病毒载体里重组加入两个AAV的ITR，然后在ITR之间插入全长抗体基因序列，病毒感染靶细胞后，抗体基因可以在宿主染色体外形成环形中间体独立表达。
- we insert the two AAV ITR in the adenovirus vector and then inserted full-length antibody genes sequences between the ITRs.When viral infect of target cells , antibody genes can form the ring outside the host chromosome and independent expression .
- 但携带AAVITR的新型Ad/AAV嵌合病毒的抗体表达量比较恒定，一直在110ng/ml左右，westernblot显示其轻重链表达量相匹配，说明表达的抗体具有生物活性。
- But with AAV ITR ’ s , the new Ad / AAV hybrid virus ’ s express the antibodies relatively constant , around 110ng / ml , Western Blot shows the expression of its light and heavy chain are matched .